What is the best ITS primers for discrimination of fungi species?

Even though ribosomal ITS as a universal barcode marker for fungi is still hampered by a few limitations, the ITS will remain as the key choice for fungal identification. I usually see the ITS 1F...

11 August 2020 3,597 10 View

How can I prepare Lysozyme digestion buffer for Invitrogen DNA extraction kit (Gram positive bacteria)?

I'd like to prepare a Lysozyme and Lysozyme Digestion Buffer (25mM Tris-HCL, pH 8.0, 2.5mM EDTA, 1% Triton X-100) for bacterial cell lysate with a final Lysozyme concentration of 20mg/mL. I need...

29 July 2020 2,818 2 View

How bin size affects Particle Size Distribution?

Hi, I have question which I could not find an appropriate answer. By default, each laser diffraction instrument plots PSD using different bin sizes on the x-axis. This means, using default...

21 October 2019 6,899 3 View

Any guidelines for number of cores/problem size in parallel CFD simulations?

Hello, I constantly submit parallel jobs in a shared HPC cluster for my OpenFOAM flow simulations and like everyone else, I have to decide how many cores I need. Our cluster runs constantly over...

11 May 2019 8,329 6 View

How can I use PCR without genomic extraction on N2a cells?

I have primers and i think i have to lysis the N2a cells but after that i couldn't find any protocol like how much i must add for template or other things.

06 June 2017 8,442 3 View

How can we draw or get the centerline of a surface using Solidworks?

I would like to learn drawing or getting centerline of a surface in Solidworks. I search on internet but I found limited information. I found a programs like VMTKLab.There is a section for...

12 May 2015 2,748 7 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does any one can help me choose a reasonable basis set for intermediate metal ions interactions with guanine (i.e Fe2+, Mn2+, Cu2+ and exc.)?

Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...

01 March 2021 6,187 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Microstrip antenna design tutorial of HFSS 3D layout module ?

I have designed a 24 GHz rectangular antenna on HFSS 3D model and got desired results while desing the same antenna with same dimensions I am getting resonance frequency at 27 GHz I am...

28 February 2021 4,426 2 View

What is Flat Lens Antenna?

28 February 2021 5,433 3 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

Compact antenna for the frequency range 1 MHz-5 MHz?

Hello everybody! I have a problem with developing a compact antenna for the 1MHz-5MHz frequency range. Tell me what type of antenna can be used and what books and articles to read to perform this...

27 February 2021 7,710 4 View

Can impedance of antenna be less than 50 ohms?

I am simulating helix antenna in hfss, right now, giving excitation with 50 ohms port. When I see the results of Z parameter (real). It shows lower than 50 ohms. Do not understand, if it can be...

27 February 2021 4,218 7 View

Should we reduce the standard unit of length to 0.31951540592 meter ?

The reasoning behind this question is to align the ratios of the proton potential in energy per charge to the speed of light in distance per time. As these two dimensions appear to be related,...

27 February 2021 947 7 View