Hello
I am about to start a research related with accumulation of high value products in microalgae and my main concern is the detection/quantification of the products since the company I am working in doesn't have analytical equipment.
So I wanted to ask if anybody knows about a simple method to detect the accumulation of PUFA's that can be used in the early stages of the research.
We know that at some point we will need to use the GC or other precise method to determine what's the real response of the algae; but in the mean while it will be useful to have an alternative method to follow the progress of the tests or to realize if the algae is having or not a positive response.
Thanks in advance for any help.
Dear Lorena
Please find my patent publication
http://patents.justia.com/patent/20150267145
Allows direct transestrification of Phospholipids and neutral lipids
Folch et al., 1958 aNd and then Christy et al., 2003 for transmetation ... And then gas cromatography. Check my paper https://www.researchgate.net/publication/269308052_Exportation_of_excess_feed_from_Mediterranean_fish_farms_to_local_fisheries_through_different_targeted_fish_species
Article Exportation of excess feed from Mediterranean fish farms to ...
Thank you all for your answers. The problema with GC is that I dont have an available equipment in my lab so I want an easy and practical way to follow the development of the storage in the algae. As Mrs. Reinecke says bodipy and nile red are interesting options but I dont have a fluorescence microscope neither. So for the moment I was thinking about extracting the oil with a sohxlet and then use iodine index to have an idea of the insaturation of the oil. What do you think??
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