Im a bachelor degree student and I am making my final year project about effect of edible coating based incorporated with aloe vera on the shelf life of fresh cut apple. i preserved the apple for 9 days. my question is i want to test for vitamin C, antioxidant activity and total phenolic content of the apple for each day. So what type of simple extraction that i can use to test them? i prefer to extract the fresh sample and not using the drying method. thank you
foe vitamin C detection, it is better to extract the sample with metaphosphoric acid since it prevent the oxidation of vitamin C in the sample. For antioxidant and TPC determination use ethanol or methanol. Please refer the following article to get an idea.
You can follow few steps before reach to conclusions:
1. Check the parameters before the aleo vera treatment then also check after the treatment immediately (say 0 day).
2. Carry on the analysis during storage period and report as % loss of those parameters based on treated one (day 0).
3. However, keep in mind the treatment should not increase all those parameters significantly.
4. Many times, people do analysis after removing the coating. However, that way, you lose portion of the sample.
Good luck.
please take care for ascorbic acid degrdation so it is better to extract the sample with oxalic 1% acid since it prevent the oxidation of vitamin C in the sample.
Jasim Ahmed thank you for your suggestion and i will follow the steps. @Sasini Ranasighe thank you i had choose your suggestion by using metaphopshoric acid..
- Badges
- Science method
- Similar topics
- Biological Science
- Ecology