SignalR real Time chat?

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Optimization of tetragonal structure ?

Hi all, What are the steps to optimizing a tetragonal structure using Wien2k code?

30 March 2024 9,205 0 View

Good greeting What are the steps to estimate the panel svar model in EViews 13?

good greeting What are the steps to estimate the panel svar model in EViews 13?

16 March 2024 9,251 1 View

Are increased LOD and LOQ values normal using LC-MS/MS?

Plant extracts were quantified utilizing LC-MS/MS in conjunction with SIM (selected ion monitoring). I anticipated obtaining extremely low values since the LOQ represents the lowest detectable...

29 January 2024 2,500 5 View

How to remove 293T Phoenix Cells from my fibroblast culture?

I had a miscommunication with a coworker, and as a result I put unfiltered virus harvested from 293T Phoenix media on to my primary fibroblasts. The transduction worked well, but now I have a...

20 November 2023 4,537 1 View

How i can download sequences from MLST C. albicans database?

I need to download the sequence of seven housekeeping genes (i.e., AAT1a, ACC1, ADP1, MPIb, SYA1, VPS13, and ZWF1b) of ST C. albicans to do phylogeny tree

18 October 2023 5,304 4 View

Dear All, kindly I want two protocols: 1. Polymorphonuclear leukocytes (PMNs or neutrophils) extraction from blood. 2. Degranulation of PMNs?

The objective: To compare the antimicrobial peptides compositions in neutrophils with stimulation and without stimulation.

19 September 2023 1,621 2 View

Hello, How to test the mechanism of action (biochemical point of view) of a anticancer-approved agent?

there are many agents approved to have anticancer agent using MTT or any other type of tests but yet the mechanism of how they induce anti proliferation or apoptotic activity is not reveled, So...

19 March 2023 2,169 3 View

Will dermal fibroblasts migrate through a 3 uM pore transwell mesh?

I'm trying to perform an experiment where keratinocytes and fibroblasts have paracrine signaling between them, but I want to keep the two cell groups separate and prevent migration. My current...

08 March 2023 2,322 0 View

How to improve the RNA concentration extracted from frozen human plasma samples ( not stored in trizol) (stored at -80)? Share your protocol if any?

I have extracted RNA from frozen human plasma samples by thermoscientific genejet RNA purification kit but of very poor quality. How to improve this extraction to run PCR for that. Attaching the...

23 January 2023 7,516 5 View

How to deploy the algorithm of psychoacoustic model MPEG 1?

I want to perform the psychoacoustic model of MPEG 1 to obtain the global masking threshold of a speech signal. Assuming that the spesch signal frame is of length N, i want to get the...

14 August 2022 7,565 0 View

Are there any instruments for studying time similar to the way it is in space?

There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...

06 August 2024 7,102 0 View

Why does the MFDFA algorithm need to calculate the profile of the time series?

As described in the Multifractal detrended fluctuation analysis (MFDFA) algorithm, it at first calculates the profile of the time series, and then other steps are operated on the profile....

05 August 2024 9,366 2 View

How can i do multivariate Time Series forecast using MLP, ANFIS and LSTM?

I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.

05 August 2024 2,977 3 View

Is there any machine to do real time pcr?

I want to know how do you make real time pcr solation ? is there any machine to make it? thanks for answering

05 August 2024 1,660 0 View

Need help with my research project on open source SIEM and machine learning?

Hello everyone, I am currently working on a research project that aims to integrate machine learning techniques into an open source SIEM tool to automate the creation of security use cases from...

04 August 2024 3,196 2 View

What do you consider to be the most relevant elements of EEG for studying cognitive biases?

I've seen articles that primarily focus on alpha and beta activity in the frontal regions, but these studies often compare healthy subjects with those having various pathologies. I haven't seen a...

31 July 2024 7,259 1 View

Where to find a gene list for CRISPRa/i library screening of regulatory factors that affect pathogenic Th17 differentiation in PBMC?

I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...

31 July 2024 2,150 0 View

How to add Cr parameter in autodock?

When I run autogrid4 it says: autogrid4: ERROR: Unknown receptor type: "Cr" -- Add parameters for it to the parameter library first! Look forward to your reply.Thank you so much!

29 July 2024 488 0 View

Is space-time dilation conceptually equivalent to space-time expansion?

Relativistic space-time is described as a four-dimensional continuum comprising three dimensions of space and one dimension of time. In this framework, space and time are interwoven, forming an...

29 July 2024 5,365 2 View

I need the housing or real estate prices data since 1950 till date in India. Can anyone guide how can I get this data ?

I didn't find any data source for continuous time series since 1950. NHB Residex and RBI give data from 2013 and 2010 onwards. Please guide. Thanks !!!

27 July 2024 6,271 5 View

Ibrahim A. Althamary

Dear Kl Wafa

May be this will help: https://www.codeproject.com/Articles/524066/SignalR-Simple-Chat-Application-in-Csharp

https://github.com/SignalR/SignalR

Stackoverflow is good for these kind of questions.

https://stackoverflow.com/

Kl Wafa

Thank you Dr Ibrahim

Salah Albermany

Dear Wafa,

https://pusher.com/tutorials/chat-aspnet/

thanks

Thanks Dr Salah