Dear all,
I am doing Vitamin D derivatization with PTAD like described in this paper Article Derivatization methods for LC-MS analysis of endogenous compounds
(reaction mechanism is in Figure 3) to enhance sensitivity in LC-MS.Normally this reaction is done in absence of water and works great, but completely drying it is a pain, so the ability to do the reaction in presence of 10% water (90% ACN) would be great.
So I tried and could observe close to complete disapperance of the unmodified vitamin D species in the presence of PTAD (not in the absence of PTAD). But, the normal modified form is only present in very minor amount, so there must be another reaction. Does anyone has a clue, what could be this side-reaction? Unfortunately, I have only a Triple-Quad, so I cannot really look unbiased with HR-MS.
Hi Florían,
have a look here:
Article Triazolinediones as Highly Enabling Synthetic Tools
Article Metabolic profiling of major vitamin D metabolites using Die...
Article The role of mass spectrometry in the analysis of vitamin D compounds
Regards
Markus
Sorry Rawaa,
but what does your answer have to do with the question? Florian wanted an answer regarding the side reactions that occur when vitamin D is derivatised with PTAD. So in future, please first read the question, understand it and if you think you have something to contribute, then answer. Thank you very much.
Regards
Markus
Hi, Florian!
How are you?
I'm from Brazil and trying use PTAD to vitamin D derivatization. Do you have some news about your question?
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