cells plated
I'm just curious if blocking peptides can be used in this fashion? Does the conjugation of the blocking peptide with the primary antibody stop the immunoreactivity with say a flouro-tagged...
31 December 2015 5,676 5 View
i think my taqman primer / probe is contaminated. is there any way to decontaminate? e.g., uv light, etc.
08 September 2015 271 2 View
I'm looking to do a time course realtime-glo vitality assay on MDA-MB-231 in a 96-well plate. I plan on doing 4 different seeding concentrations to assess linearity of signal, however there is...
05 June 2015 1,888 4 View
I'm looking to scale up my siRNA reverse transfections from a 6-well to 384-well plate for adherent SH-SY5Y. Should I be scaling the siRNA concentration by surface area in the well, or by the...
31 December 2014 4,874 6 View
I'm performing reverse transfection on SH-SY5Y using Viromer Green, and I get wonderful transfection efficiency (90+%). See picture (RFP labeled siRNA fluorescence overlapped on phase contrast...
10 November 2014 7,589 7 View
We are electroporating a pcDNA6.2 plasmid contain miR RNAi constructs into SH-SY5Y. I plan on selecting these cells with blasticidin, and the literature suggests closer to the 10ug/ml mark for...
09 October 2014 6,550 3 View
I thoroughly pipette my cells prior to electroporating so they should form a monolayer when plated. Though every time I electroporate, they clump. I'm not entirely sure why. When I passage, they...
08 September 2014 6,425 3 View
What is a good number of SH-SY5Y to freeze down for long term storage between 1-12mo? Ideally, I'd like to initially plate in something larger like a t75 or t150, though I'm not sure if it is...
06 July 2014 4,850 2 View
How do people typically go about electroporating differentiated SH-SY5Y? I was thinking that you would have to electroporate them first then recover in media supplemented with retinoic acid. Would...
06 July 2014 1,798 1 View
Has anyone had any experience using CPPs to transfect small plasmids or siRNA into primary human neuron? I'm just curious to see if you would share your experiences. I'm going to be testing with...
06 July 2014 1,498 2 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
I have face this problem anyone help me how to solve this issue ?which is below Fatal error: There are inconsistent shifts over periodic boundaries in a molecule type consisting of 78 atoms. The...
07 August 2024 2,598 1 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...
04 August 2024 5,228 4 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
