I have a fairly simple question:
Is it better to consistently use tissue from a single hemisphere for one type of analysis? For example, if you are doing both immunostaining and RNAscope, should you only use right hemisphere for immunostaining and left for RNAscope? Or is it better to collect mixed hemisphere tissue for both techniques?
I guess ultimately, is there any hemisphere lateralization that should be considered for performing analysis of tissue? If there is any hemisphere bias is it severe?
Thank you!
It might be better to use both spheres for each of your experiments; replicate them in Triplicates and check if there is any lateral bias in your results. Not only would that give you better clarity regarding your data (in case hemisphere lateralization does occur), it would also reduce any pre-conceived bias regarding the data concerning lateralization, especially during peer-review.
Shalini sanyal is right. However,due to limited number of samples it does not practically possible in most of experiments. Using mix of hemispheres for both techniques may reduce the lateralization bias.
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