Hi I am depleting the ribosomal RNA of my samples with ribominus kit for ion proton libraries. After running a pico chip in the bioanalyzer I see a clear depletion of the main 18s and 28s (just look at sample 3). However in the bioanalyzer "gel" image I have a very clear low weight black band. I dont know if it should look like that or just not bands. Also I dont know if the black band correspond to the 5s....
Thanks in advance
In my opinion, what you see is the lower marker of the gel dye, which somehow has been misplaced by the software. If you go to your electrophoregrams, check if the lower marker is not set to a small "peak" (even the smallest visible bump in the FU curve) before your strong band and then reset it to the "normal" position. Also, I think your RNA ladder needs changing, it is partially degraded and should have a better "look".
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