Should I storage DPPC liposomes above or below their melting temperature (Tm)?

Dear Natalia A. Kasian

Storage not only depend on Tm

http://www.tandfonline.com/doi/abs/10.3109/08982109909035549

Liposomes of DPPC have been determined to have a transition temperature of 41.3 ◦C = (Tm=41.2 °C).

Since DPPC has a phase transition temperature of 41 C, a 60 C water bath was needed to hydrate DPPC with same volume of buffer A. Before extrusion, the extruder was also heated to 60 C. Then the hydrated DPPC lipids were extruded at 60 C for the whole extrusion process. In: attached article

http://www.rsc.org/suppdata/c5/nr/c5nr04805b/c5nr04805b1.pdf

Liposomes remained stable in liquid form at for at least 6 months when the drug-to-lipid molar ratio was below 15% in: https://www.hindawi.com/journals/jdd/2011/629234/

Pls. also find the attached files

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1304279/

Page 155 in: Whateley. 1992. Microencapsulation of Drugs. CRC Press, Pp.309. ISBN 3718652471, 9783718652471

Otto Braun-Falco, Hans C. Korting, Howard I. Maibach. 2012. Liposome Dermatics: Griesbach Conference. Springer Science & Business Media,Pp.364. ISBN 3642483917, 9783642483912

https://www.researchgate.net/profile/Janelle_Lauer/publication/5762872_Effects_of_Drug_Hydrophobicity_on_Liposomal_Stability/links/56bb11d808ae3af6847e2abe.pdf

http://www.sciencedirect.com/science/article/pii/S000527360600068X?np=y

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC392531/pdf/pnas00017-0210.pdf

https://avantilipids.com/tech-support/liposome-preparation/

http://www.rpgroup.caltech.edu/courses/aph161/Handouts/keller1.pdf

http://www.pci.uni-heidelberg.de/bpc2/pdfs/paper2011/Schubert2011.pdf

Hoping this will be helpful

Regards

Prof. Houda Kawas

Article Effects of Drug Hydrophobicity on Liposomal Stability

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