I would be grateful if you can help me in clearing my doubt how I do characterize the biosurfactant produced (whether I should opt for LC-MS or GC-MS). Can you please help me with the protocol?
LCMS and HPLC are good for the analysis of biosurfactants. GC is good if you are interested to see the fatty acid composition of the biosurfactant. Example, rhamnolipids have fatty acids, by derivatizing the methylesters of the corresponding fatty acids; we can analyze them to see the carbon number, unsaturation etc in GC. LCMS and HPLC are the best tools; if the surfactant is new then you need to do NMR and x-crystalography to reveal their chemical nature. The review by Prof. Banat (sent by Zulfiqar) is a informative article with detailed methodology.
LCMS is good option for complete characterization of biosurfactant.
Could you send me an informative article with detailed methodology?@Thavasi Renga Thavasi
Best regards,
Rifky
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