what are the opportunity of determination of embryo sex in the fertilized egg of chicken
PCR with primers for male-specific genes/variants e.g. SRY, amelogenin can be used.
The ratio of male: female hatch on domestic chicken eggs is almost 50:50. There are several sex determination methods you can use, depending on whether the fertilized egg is incubated or not.
If incubated, cytogenetic or hormonal analysis is possible. If un-incubated, could be by morphometric, hormonal, or molecular analysis. So far, flow cytometry of cellular DNA derived from peripheral blastoderm cells of not incubated eggs is considered the reliable one.
I recommend this article (https://doi.org/10.1017/S0043933908000111), it is quite good at reviewing existing methods.
In the chicken and other birds, sex is determined by a ZZ:ZW sex chromosome system. ... While the genetic trigger for sex determination in birds remains unknown, some promising candidate genes have recently emerged. The Z-linked gene, DMRT1, supports the Z-dosage model of avian sex determination Rafea M.T Khaleel
Since sex in poultry is determined by the female, eggs contain either a chromosome
X or Y
It is best and easier than differentiate the sex of chicks after hatching we can hatches only females eggs if we want layers or males if we want broiler
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