We are a group of students relatively new in the research world. We are currently working on our research assessing the anti-carcinogenic effects of active compounds present in Colocasia esculenta parts in mitigating prostate cancer carcinogenesis. This study aims to utilize the whole Colocasia esculenta plant, extracting and isolating the active compounds present in its roots, leaves, and stem, and assess and compare their anti-carcinogenic effectivity against prostate cancer. Thus, we want to ask experts, preferably taro experts but can be also general experts about chemoprevention and oncology, out there several questions below regarding research design and concerns.

  • What statistical methodologies are used to utilize and measure the efficacy of the taro's anti-carcinogenic activity?
  • We've encountered several studies that use crude TE in evaluating the taro's effects against cancer cell lines. And we're currently applying this to our research. We want to ask if wild taro extract can serve as a doable surrogate for the poi extract. Moreover, we also want to know if the taro (raw or cooked) condition could produce a mixed result concerning taro's inhibitory effect against carcinogenesis?
  • Our study involves testing crude TE taken from various parts of the plant on prostate cancer cell lines. When testing out the carcinogenic and migration assays of obtained prostate cancer cell lines, generally, how much isolated Colocasia esculenta phytochemical extract is needed for experimentation to assure maximum data accuracy?
  • Do you see the potential of taro's leaves and stem from having anti-carcinogenic properties aside from the corm? Are active compounds of the corm of C.esculenta might be more less-efficient in mitigating carcinogenesis than of the leaves and stem?
  • Can the inhibitory effect of Colocasia esculenta (taro) extract against carcinogenesis be more pronounced in vitro than in vivo? Or in the other way?
  • On the matter of sample toxicity, what should we worry about when obtaining Colocasia esculenta phytochemical extracts?
  • The processes we found viable for active compound extraction are maceration and decoction. Would you recommend these too, or are there other more efficient techniques?
  • Are there any dangers that we should expect/watch out for from acquired taro extracts? If so, what are these?
  • We've encountered several studies that use crude TE with more-or-less similar results as yours. And we're currently applying this to our research. We want to ask if wild taro extract can serve as a doable surrogate for the poi extract. Moreover, we also want to know if the taro (raw or cooked) condition could produce a mixed result concerning taro's inhibitory effect against carcinogenesis?
  • Our study directly involves testing taro extract into human PCa cancer cell lines. In your article, you used a rat colon cancer cell line first. Thus, is it appropriate to start experimenting first on human cancer cell lines rather than on rat cancer cell lines beforehand?
  • Are there any additional measures we need to take to attain the most accurate outcomes possible? Anything we need to take note of or remember?
  • Overall, does this research seem feasible enough to be accomplished by us students who are only in high school?
  • We are willing to hold a video consultation session if possible to address such concerns. Your help is very much appreciated.

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