Guys I have a small Question. Sequencing results indicates that the dna is from fish and in real the sequence was from a human. What could be the reason for it?
Contamination during library prep? If your workflow involved PCR steps, there's a chance any contaminating fish template could have been amplified up enough to be represented in your sequencing reads
Does your lab also work with fish specimens? Did you have a tuna sandwich for lunch that day?
Haha Thanks for the reply. But I did not had tuna sandwich that day and The sample was pure and not contaminated.
IN BLAST select Human Genome. Hope you'll get some hits.
I think Polina is probably correct . If the sequence is very similar between species and the change is just a polymorphism then maybe you have found a rare polymorphism in humans that is just the same as the normal sequence in fish particularly if the change does not change the protein sequence
Maybe if you used a seq. facility they accidentally swapped the samples. Should not happen, but you never know. How many of your reads blast to the fish?
This is interesting. We have sequenced some salmon fish DNA sequence from a tobacco plant derived from a protoplast transformation project. The salmon DNA seemed to insert into a locus we are studying. We did use salmon DNA as a carrier during transformation. We are wonder whether this salmon fish DNA is derived from the carrier DNA we used. Have you ever used 'salmon fish DNA' for anything in your human cell project??
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