Comparing the constructs by using different scales of measurement in different samples?

I was wondering whether it would be possible to measure the same construct (i.e. the perception of training) in two samples by using different scales for each sample (i.e. employed and unemployed...

02 May 2020 3,917 10 View

How to culture cells before alizarin red staining.?

08 May 2017 5,630 7 View

Are there any (high quality) potential energy surfaces for singlet electronic excited states of hydrogen sulfide (H2S) available?

22 February 2016 3,428 3 View

Is Qiagen AllPrep DNA/RNA FFPE kit compatible with Laser Capture Micro-dissection using arcturus capsure HS LCM caps?

08 March 2015 1,823 2 View

Any ideas on dose-response inconsistency in XTT cell proliferation assay for positive constrols?

So, I test plant extracts for their cytotoxicity effect using XTT cell proliferation assay kit. As positive controls vincristine and docetaxel are used. The problem is I can't observe dose...

14 December 2014 2,183 2 View

Can one buy/procure a nanobody plasmid library for phage display

I would like to make a phage display using a synthetic or naive nanobody library in order to identify nanobodies targeting different neuronal proteins. However, I am not able to find a provider...

01 January 1970 3,258 4 View

Salinity treatment and species "nested" within light treatments - GLM or ANOVA?

Hello. I have an outdoor experiment with 2 species, 5 salinity levels and 2 light treatments (50% and 100% daylight). To produce the light treatments we used shade cloth and for practical reasons...

01 January 1970 9,979 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

Which is the most convenient manufacturing software for Additive Manufacturing?

Which tools can be used to generate machine codes for additive manufacturing?

02 March 2021 7,220 2 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Does different dilution factors affect negatively on NH3 reading ?

I have a sample of treated water sample & asked to obtain ammonia conc. in sample using a machine with 5-25 ppm detection range. However, upon testing with different dilution factors, all...

27 February 2021 8,483 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View