Sequencing or Sequencers?

More Emil Arvedsen's questions See All

How to Dedust Activated Carbon Granules?

Dear all, We are producing activated carbon from hazelnut shells but have an issue about the dust of the a.c. granules also known as the dust film on granules. Linear vibrating seives doesn't do...

11 July 2024 9,782 2 View

How come I get an inverse correlation signal output in my ELISA titration?

Hello, I am identifying nanobodies for my protein of interest. I have identified several different nanobodies showing specificity towards my target and conducted a validation assay (ELISA...

01 December 2023 6,881 1 View

What is the best method to measure tourism carrying capacity in national parks or other large natural regions (mountain areas, etc.)?

In your opinion, what is the best method to measure tourism carrying capacity in national parks or other large natural regions (mountain areas, etc.)? What objective criteria can be used to...

19 October 2023 9,457 0 View

What methods can be used to enhance or improve quality control?

Is there any methods that can help quality control enhance its role?

03 September 2023 8,310 1 View

Mini prep plasmid with DNA contamination? How can I avoid DNA contamination trying to QuikChange mutate my plasmid?

Hello, I am currently working on making mutations in my plasmid conducting Quikchange. The plasmid size is 3800bp and I am using primers with several mutations. However, both my parental plasmid...

19 July 2023 5,156 3 View

What are the key differences and benefits of using Seurat and Scanpy for single-cell analysis?

Dear ResearchGate Community, I am currently engaged in single-cell analysis for my research project and would greatly appreciate your insights and experiences regarding the use of Seurat and...

19 June 2023 9,045 5 View

What is the negative controls in phage display (biopanning) and troubleshooting panning protocol?

Hi there, I am currently panning against GFP and pre-depleting with PBS+0.5%BSA using the steps attached (modified from Barbas's protocol). GFP and BSA are immobilised on high-binding microtiter...

24 January 2023 388 3 View

How can i determine the term symbol for spin orbit coupling from this Orca Output?

I performed ORCA SOC CASSCF calculations on the Molecule AsCH3+. Without spin orbit coupling the mullikan term symbol should be 2A'', since due to ionization of an electron the c3v symmetry is...

01 November 2022 8,613 1 View

Protein with aldehyde tag disappears on VivaSpin (PES) MWCO 5000?

We are working with a protein with a MW of 12 kDa with a C-terminal "aldehyde-tag" (formyl-glycine) produced as described in: https://pubmed.ncbi.nlm.nih.gov/17450134/ After purification and...

16 October 2022 9,658 0 View

Are there any suppliers for AM grade Waspaloy powder in India?

I m looking your inert gas atomized AM grade Waspaloy powder suppliers in India.

19 June 2022 1,997 0 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Is there any machine to do real time pcr?

I want to know how do you make real time pcr solation ? is there any machine to make it? thanks for answering

05 August 2024 1,660 0 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Is it necessary to attach fluorophore at 3' end in MIC RT PCR machine for Dual hybridization Probe designed especially for genotyping assay?

I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...

28 July 2024 5,658 0 View

Are these cassettes suitable for expressing PETase mutant in E. coli?

I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...

28 July 2024 7,559 1 View

Mohamed-Mourad Lafifi

Dear Emil Arvedsen,

I suggest you to see links and attached files on topic.

https://www.genome.gov/about-genomics/fact-sheets/Sequencing-Human-Genome-cost

https://www.ncbi.nlm.nih.gov/books/NBK26837/

https://www.bio-rad.com/webroot/web/pdf/lse/literature/1665027.pdf

https://labtestsonline.org/dna-sequencing

https://worldwide.promega.com/resources/guides/nucleic-acid-analysis/dna-purification/

Best regards

Abdelghani Rouini

Please read this article

The sequence of sequencers: The history of sequencing DNA

James M. Heather and Benjamin Chain

And also this link:

Top 10 Sequencing Companies

https://www.genengnews.com/a-lists/top-10-sequencing-companies-2/