Hey Experts,
I am planning to buy LC detector for lipid analysis. Can you please suggest me which LC detector would be best for lipid analysis? I am using SCIEX 5500 QTRAP and Shimadzu LC.
Thank you in advance
I suggest getting a diode array detector to put between the Shimadzu LC and the Sciex 5500 Q-TRAP. If you are planning to use the detector as a stand alone unit, separate from the mass spectrometer, then get an evaporative light scattering detector.
If you are using a QTRAP why do you need another detector ? Generally lipids are hard to detect with LC detectors due to poor absorbance characteristics and no fluorescence (without derivatization). Juat use the QTRAP to measure lipids - it will be much easier.
the best ditector for HPLC is the refractive index (RI) detector because is the only universal detector for HPLC
I think so
good luck
*Mohamed's comment above is misleading and inaccurate.
There is no such thing as a true Universal Detector for HPLC (If there was one, RID would not be it.). RID is close and sometimes you will find misinformation about RID being "universal" (especially in vendor product literature) because it only relies on one physical property for detection (refractive index). However, as your sample's RI approaches the RI of the mobile phase, the sensitivity gets lower until it is finally zero. So that is not a Universal detector at all.
For lipid analysis, there are thousands of papers and articles showing the use of RID, ELSD, CAD and MS units for detection. ELSD is by far the most popular and RID is the simplest to use, least expensive and most limiting (very poor sensitivity).
Try a web search for example application notes and papers.
I've been working with FAME analysis by GC-MS for the past 4 years and recently tried to run some samples in an UHPLC-CAD. I got stunning results since I finally could get more information (DAG, TAG,...) about my samples.
Find attached a (not fair) comparison between UV (blue and pink chromatograms) and Charged Aerosol Detection (black chromatogram) for Lipid Analysis. We're currently developing this method and I can't share the protocols because we didn't establish one, although you can find a Thermo Fisher Literature on Lipid Analysis by HPLC-CAD on the web.
If your QTRAP is not intended to run in your lab's routine, I would advise you to get a CAD and use the QTRAP for sample confirmation. If you're planning to do some Lipidomics you should keep your MS method development!
I hope this material may help you!
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