Hello everyone,
I am a new PhD student starting a project on microRNAs (miRNAs) in myocardial infarction (MI) patients. I would greatly appreciate any guidance on the following points to ensure proper sample collection, storage, and analysis:
Your guidance will be instrumental in helping me get started on my PhD project.
1. EDTA Tube would be good for price wise, Tempus (Thermo) or RNA Shield (Zymo Research) for better stability
2. EDTA, not more than 4 hours after collection and storage in 4C, Tempus (Claimed 5 days in 4C) and RNA Shield(Claimed 1 month in ambient temp)
3. Recommended storage is -80C, but if you want to isolate it from different part of the blood, separate it first before freezing
4. refer to this article https://doi.org/10.11613%2FBM.2021.030705
5. Yes you can, for how you should aliquot, need to determine first how much is needed for each of the test then calculate the rest by your self and aliquot accordingly
6. Yes -80 is a must, the buffer is Citrate/TE or commercially available RNA storage buffer
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