I am working with a protein and trying to resolve it on Blue-Native PAGE. Can somebody suggest be a recipe for it?

I have been trying to perform Blue-Native PAGE using procedure from doi:10.1038/nprot.2006.62. However, the stacking gel does not solidifies even after two hours, at room temperature. Can...

04 April 2019 4,777 2 View

I am planning for Native PAGE for a protein with pI 8.5 - 8.7. What should be the minimum difference of pH of buffer to avoid electrode switching?

The Gel has pH 8.9. and the pH of loading dye is 6.67. What should be the pH of running buffer to allow smooth running of my protein into the gel. I prepared buffer of pH 8.49 but I was unable to...

25 March 2019 9,877 6 View

Any suggestion what's going on?

I submitted a manuscript on a novel and interesting finding on July 30, 2018 to a reputed journal. Following editorial review it went to peer-review. It is over 8 months, no decision yet. What...

24 March 2019 9,737 2 View

How to find the natural frequency of fixed thick glass?

Dear researchers, i am looking for equation to find the natural frequency for fixed thick glass. please suggest

28 November 2018 2,736 2 View

What are the advantages growth of GaN on graphene in optoelectronics?

I would like to write my research plan for my PhD on this topic.

21 April 2015 4,301 3 View

Is it compulsory/mandatory to examine the mediating effect in the conceptual framework that was analyzed utilizing AMOS?

Increased value of invertebrates - Mediating variable

08 April 2015 2,834 5 View

How is oil content estimated in mustard seed? Can anyone provide me a protocol regarding this?

I want to estimate the percent of oil present in mustard seed.

24 March 2015 2,233 7 View

What is the minimum sample size needed to conduct the SEM analysis utilizing AMOS software?

05 February 2015 9,648 7 View

How to stop dye transfer from one cell to another?

I am trying to optimize ADCC for HIV infected cells. I am labeling my targets with CFSE and any other membrane or nuclear dye. My effectors are unlabeled. The problem is the second dye, no matter...

15 January 2014 7,731 3 View

What is the scope of ethnobotanicals in the poultry nutrition?

Ethnobotanicals means herbs that are used by the natives for long time to treat differnet health issues. However some time there is no scientific justification available.

16 April 2013 7,103 2 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

How can i resolve the "final solutuin is not converged" error in Comsol for my heat transfer simulation??

I have been trying to simulate heat transfer problem in Comsol 5.5 for long time but it always throw an error stating: "Failed to find a solution. Maximum number of Newton iterations...

28 February 2021 8,626 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

How to approach CFD simulation results of a flow past a cylinder?

Hi all, I'm doing a transient simulation of flow past a rectangular cylinder close to the free surface. As far as I'm concerned, the common practice is to discard the initial stages of the...

25 February 2021 1,348 3 View

Error in comsol?

Dear respected researchers, while performing CFD analysis in Comsol it's showing like this Error: Nonlinear solver did not converge. The maximum number of Newton iterations reached can...

24 February 2021 249 3 View

How to constrain genetic algorithm input u?

My real system (buck converter) can only take an input of 0 to 1 (duty ratio) and I need to constrain the system so the controller action keeps within this bounds. How can I do this? The code for...

22 February 2021 2,125 3 View

How to overcome convergence issues in concrete arch models?

Hi, I have modelled a three-span arch bridge with soil fill over along with pedestals. The FE modelling is done in LUSAS with HX8M solid elements (i.e. 3D solid modelling). The non-linearity is...

20 February 2021 1,346 2 View

Comsol Maximum number of Newton iterations reached?

Comsol is investigating deformation due to gravity. Gravity is applied to the nanosheets to check for displacement and stress. If I input the Young's modulus of the channel to about 1 [GPa], the...

15 February 2021 6,027 3 View