I am working on Genome editing. I have target some genes of potato plants using CRISPR Cas system. Some of my plants did not develop roots on selection that is on kana but while screening I came across that some of the plants show the presence of Cas 9, the PCR is set for the presence of Cas 9 system. What is the reason?
If this is so then does I have to screen all the plants that have not even developed roots on selection?