Arsh Bibi

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Questions related from Arsh Bibi

How to identify Phytophthora infestans??

I have collected some leaves samples from potato fields showing late blight symptoms, grown them on PDA media ( that was the only available media I have). I am trying to isolate them...

17 March 2022 7,228 4 View

Screening the plants (potato) that have not develop roots on selection ( Kana) but give a clear band of Cas 9 presence what is the reason?

I am working on Genome editing. I have target some genes of potato plants using CRISPR Cas system. Some of my plants did not develop roots on selection that is on kana but while screening I came...

24 December 2020 1,788 3 View

RNA isolation protocol from plants with high phenolic and Polysaccharides.

I am trying to Isolate RNA from sugarcane. The objective is to study the involvement of genes in red rot diseases. Plant stems are inoculated with Red Rot fungus inoculum. Plants are mature and...

01 January 1970 6,240 3 View

How to identify Phytophthora infestans??

I am working on P infestans for the last 3 months as I collected leaf samples showing symptoms of late blight from different regions of Pakistan. Placed them on PDA media to isolate P.infestans,...

01 January 1970 8,706 1 View