Samples collected in EDTA tubes will result in low zinc level but our results are increased why?

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Matthew Wheal

First thought: Rubber gloves. Latex and or powdered gloves are made with as much as1% Zn and are often coated in Zn-based powders for wearer comfort (but analysts nightmares.)

See the article at: Garcon et al 2017 Nitrile, Latex, Neoprene and Vinyl Gloves: A Primary Source of Contamination for Trace Element and Zn Isotopic Analyses in Geological and Biological Samples. Geostandards and Geoanalytical Research vol 41 issue 3 p367-380. doi: 10.1111/ggr.12161

Zinc is everywhere and it takes considerable effort to remove it from your protocols to a point where you can get accurate results. Talk with your institution's metals analyst and see what they can recommend.

Go through every step to see where you touch the sample, every container used, sample vial, cap of sample vial, pipette tip, (and previous solutions used with that pipette) etc. If you grasp the tip with powdery gloves, Zn will go all the way through your sample and contaminate it. Very frustrating.

Did you prepare blanks in the same collection tubes to see if they were clean of zinc? Not just water or saline, but a 1% HNO3 solution to properly leach the tubes. You might have just hit a bad batch, or it could be consistent throughout the production of the tubes.

Best of luck.

Manal Sameeh

If your laboratory results are showing increased zinc levels despite collecting samples in EDTA tubes, there could be several potential reasons for this discrepancy:

Contamination: If there was any contamination during the collection, handling, or analysis of the samples, it could lead to erroneous results. Zinc can be present in various materials used in the collection and analysis process, and even trace amounts of contamination can affect the results.

Analytical Methodology: The method used to measure zinc levels in the samples may not be suitable for samples collected in EDTA tubes. Different sample types may require different analytical methods, and using the wrong method can yield inaccurate results.

Interference: EDTA, the anticoagulant used in the tubes, can sometimes interfere with certain laboratory tests, including those used to measure zinc levels. This interference may lead to falsely elevated results.

Hemolysis: Hemolysis, the breakdown of red blood cells, can release intracellular components, including zinc, into the plasma or serum, leading to artificially increased zinc levels. Hemolysis can occur during sample collection or processing if the samples are mishandled.

Sample Storage: Improper storage of samples, especially for an extended period, can alter the composition and stability of the analytes, potentially affecting the zinc levels measured.

Specimen Collection Timing: The timing of sample collection in relation to zinc intake or metabolism can influence the results. For instance, recent zinc supplementation or dietary intake could impact the measured zinc levels.

Individual Variability: Zinc levels in the body can vary among individuals due to various factors such as age, sex, dietary habits, and underlying health conditions.

To resolve the discrepancy and ensure accurate results, it is essential to review the entire testing process, including sample collection, handling, and analytical methodology. If there are concerns about the results, it is recommended to consult with the laboratory or a qualified healthcare professional for further investigation and appropriate interpretation of the findings.

Naga Veera Yerra

Thank you!