What are the application of Fixed points thoery in Chemistry.?

It is said that fixed point theory has lot of applications not in the field of mathematics only but also in various areas. So I am looking for applications in Chemistry only..

26 February 2021 3,533 1 View

How to Interpret Partial Eta Squared Numbers For a Repeated Measures Anova?

I have obtained Partial Eta Squared Numbers through Two-Way Mixed Anova Analysis. My results for different constructs are: .105, .135, .038, .068, .061 respectively. I wanted to know, whether...

24 February 2021 7,122 1 View

Can we proceed to perform Two-way Mixed-ANOVA despite normality violations?

While testing assumptions, Shapiro-Wilk Test failed. However, I proceeded with the analysis and the interaction effect was significant. During our course- lectures, it was mentioned that Anova...

24 February 2021 228 3 View

Problem in energy minimization using Gromacs?

I have done energy minimization of DNA using Gromacs with tutorial lysozyme in water. The potential energy curve show a steep drop.

18 February 2021 4,822 2 View

Level 1 pre-post multilevel regression: Panel data or ANOVA approach?

Dear colleagues, I am currently teaching myself in multilevel analysis using educational data. I found few to zero literature on basic analysis methods. In my context we frequently use level-1...

17 February 2021 9,378 5 View

What is the best method for preparation of Polymeric nanoparticles for co-delivery?

We are planning to make Polymeric nanoparticles for co-delivery of 2 flavanoides. Which is the best method, that one should follow.

09 February 2021 7,086 8 View

How to extract RNA from brown adipose tissue(BAT)?

30 January 2021 6,610 3 View

Is there any software to generate pdb of G quadruplex?

I have a DNA sequence which has predictability to form G quadruplex . I need a software to generate a pdb of it which will form G quadruplex. I am using open Babel but I am not able to set the...

29 January 2021 6,190 4 View

What is the ACTUAL COST OF A PUBLICATION??

Hi. It's 2021 January. Recently, I got to know that Elsevier has converted around 160 journals into some "Transformative Journals" and follow the recommendations under "Plan S"...

19 January 2021 9,985 2 View

Does anybody have the recipe for making a home made R electroporation buffer for Neon transfection systems?

I tried the recipes below in two cells lines: 1.DPBS 2.DPBS+MgCl2(1mM) 3.DPBS+Sucrose(250mM) 4.DPBS+MgCl2(1mM)+Sucrose(250mM) 5.R Buffer (Neon) the results showed that R buffer is the best one in...

06 January 2021 6,776 1 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

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22 February 2021 9,932 11 View

How to locate and collect the pineal gland in the amphibian brain?

Our team has been trying to locate and collect the pineal gland in the American bullfrog's brain (Lithobates catesbeianus) for some time now. However, we don't have a standardized protocol for...

18 February 2021 3,453 4 View