05 October 2023 0 652 Report

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More Continente Elizalde-Domínguez's questions See All
What does effector:target ratio really means in CAR-T cell therapy?

Regarding CAR-T cell therapy, during co-cultures with different effector:target ratios, when we refer to "effector", does it involve the total T cells? For example, does a ratio of 5:1 mean 5...

28 May 2024 2,757 4 View

¿Piensas que una persona que ha maltratado psicológicamente y físicamente puede rehabilitarse 100%?

ESTÁN TODAS LAS PERSONAS PREPARADAS PARA UNA REHABILITACIÓN 100% EFICAZ

04 March 2024 3,344 3 View

Has anyone have taken confocal images of apoptotic cells stained with Apotracker Tetra?

Hi! I was wondering if anyone has taken confocal images of apoptotic cells stained with Apotracker Tetra, from Biolegend. There is information using Apotracker Green, but I haven´t found a...

06 February 2024 8,343 0 View

Activated & lentiviral transduced T cell FACS Sorting protocol?

Hello, has someone performed a T cell FACS Sorting after lentiviral transduction? We would like to purify those T cells that were transduced using GFP as reporter, and continue expanding the cells...

21 January 2024 2,541 0 View

Hola, ¿hay estudios o artículos sobre neuroarquitectura y música que me puedan recomendar o compartir?

Estoy interesado en informarme acerca de la relación entre la neuroarquitectura y la música. Digamos, efectos de la música en el diseño edilicio o arquitectónico, efectos en de las edificaciones y...

18 October 2023 2,789 0 View

Is polybrene necessary for lentiviral titration with HEK293T?

Hi! We are producing lentivirus for T cell transduction. However, there are several protocols that do not mention the use of polybrene for titration in HEK293T, but they use centrifugation...

03 October 2023 7,363 2 View

Type I error arising?

Dear community, I want to ask you a methodological/statistical question. We performed ten independent hierarchical linear regressions divided by gender for an article writing (N=1502...

12 September 2023 2,386 2 View

Should I need to add Il-2 to a human CD3/CD4/CD8 T cell culture to induce exhaustion?

Hi! I want to induce an in vitro exhaustion model for human T cells, purified by magnetic columns. I found a protocol in which the authors cultivate the cells with Dyna beads (I have other...

15 August 2023 6,577 0 View

How are relations about spirituality ,religion and mental health?

How are relations about spirituality ,religion and mental health?

12 August 2023 6,407 2 View

Help, what program or software can i use for calculate RMSD between two small ligands?

I need calculate RMSD between ligands

04 May 2023 4,961 3 View

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