Considering the risk of salinity by well water (in present and future), in fields small, why no viable methods to reduce damage? Being best what agricultural limestone, for example.
Hello everyone, I'm having some conceptual misunderstandings regarding non-reducing SDS-PAGE. In this situation, we omit reducing agents such DTT or BME from the loading buffer to preserve...
18 July 2023 3,667 2 View
Hello all, I am used to using the TurboBlot for Western Blot and had no issues with exception of transferring high MW proteins. That's why I wanted to try the Wet transfer method. However,...
23 June 2023 2,331 4 View
Hello all, I am studying the benefits of a diet in the abrogation of high-fat diet induced cardiac remodeling of the heart. I am studying this in C57BL/6J mice and the study was already conducted...
10 April 2023 4,034 0 View
Sorry for the odd question, but I've recently had 2 students in the lab (consecutively, not at the same time) finding completely opposite results... one found a massive reduction in I-O...
23 January 2023 9,804 4 View
We are analysing the geometric morphometrics of different populations of an orchid, including populations distributed along its geographic range. We aim to test the central-periphery hypothesis,...
16 November 2022 3,866 2 View
We don't know the sequences of the parental genomes to assess SNPs because this cell line was established years ago. I am interested in looking at the IG-DMR and Meg3-DMR of the Dlk1-Dio3 locus on...
14 November 2022 9,438 0 View
Hi community! I need some piece of advice here. I have quantified my protein and peptides samples using BCA Pierce Kit and applied 20 ug of protein per well to run the electrophoresis. The...
20 October 2022 8,088 7 View
Hello, I'm trying to figure out what are the changes to the protein structure caused by insertion of an adenine in a human gene, that causes a P->A amino acid change at the insertion site but...
12 October 2021 5,215 3 View
To measure temperatures around 3,000K, there are few options, such as optical techniques or special thermocouples, with some disadvantages. On the other hand, I am trying to use the noise...
07 April 2021 5,761 1 View
Dear all, I am meshing an annular tube inside a fluid domain (attached images). This tube has fluid flowing through it. Also, there is fluid outside of it. My problem is: I am trying to generate...
14 March 2021 2,462 10 View
Approximate concentrations are require in compared with the WHO permissible limts
11 August 2024 2,722 1 View
I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...
10 August 2024 4,003 2 View
How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?
09 August 2024 3,288 2 View
Kindly response
08 August 2024 1,212 2 View
One can try to generalize the Vandermonde determinant in the following direction: Let $A$ be any symmetric $n$-order square matrix. Consider its powers' diagonal elements $(A^k)_{ii}$ and...
08 August 2024 6,689 1 View
How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under optical microscope?
07 August 2024 2,132 3 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds
04 August 2024 3,018 3 View
Why is nonpoint source pollution potentially more harmful and difference between point and nonpoint sources of water pollution?
01 August 2024 1,180 2 View
How do living organisms play a role in the water cycle and why is nonpoint source pollution potentially more harmful than point source pollution?
01 August 2024 7,061 2 View