Is there a way to determine methylation patterns on the maternal vs paternal allele in C2C12 cells?

15 November 2022 0 9K Report

We don't know the sequences of the parental genomes to assess SNPs because this cell line was established years ago. I am interested in looking at the IG-DMR and Meg3-DMR of the Dlk1-Dio3 locus on chromosome 12 which is maternally imprinted in WT cells. Are there any established protocols that are similar to regional bisulfite-seq but can give me more information about the methylation pattern on the maternal vs paternal allele?

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