We are analysing the geometric morphometrics of different populations of an orchid, including populations distributed along its geographic range.
We aim to test the central-periphery hypothesis, which expects lower morphological diversity at the distribution edges. We were able to use MorphoJ at several steps of morphometric analysis, from organizing the landmarks to running PCA, Anova tests etc.
However, we are struggling to find out how to measure the morphological diversity of each population. I believe it is something related to a measure of dispersion of the morphological variability of the individuals from one population, comparing to the centroid of the same population. But there are other methods in the literature.
Thus, I would like to ask you if MorphoJ could be used to provide a morphological diversity estimate for each population we are analysing.
I think that you are looking for a test to compare canalization between populations. I'm right? I know two ways to do that, compare variance/covariance matrix using an ANOSIM with population levels as factor, or comparing principal components (according to the variance explained you may use 2 o 3 componentes) with a Levene test. The first method is the most used and it is suggested by several experts in geometric morphometrics, but the second one looks also reliable to me, I hope i've helped, cheers.
Hi Fabio!
No idea about MorphoJ but if you are familiar with R you could have a look at the ‘dispRity’ package https://cran.r-project.org/web/packages/dispRity/dispRity.pdf or even the 'morphol.disparity' function from the geomorph package https://www.rdocumentation.org/packages/geomorph/versions/3.0.7/topics/morphol.disparity
Cheers,
Narimane
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