RT-qPCR recommended protocol-concentrations for human brain tissue (NMDA Receptor)?

22 January 2024 1 5K Report

Is there any recommended protocol (reagents' concentrations) for RT-qPCR in human postmortem brain tissue for the identification of NR2A (NMDAR) subunit? I cannot find neither a protocol let alone ranging concentrations.

Sung-Jun Lee

I have a protocol for mouse brain tissue and NR2A. Would this work for you?

Badges
Science topic

More Vasiliki Tempeli's questions See All

Hello, I have a problem with the FLAC 2D . It appears an error "cannot open the file.." Why?

I'm starting to write a code in FLAC 2D and suddenly appears a message "cannot open the file" and I don't know why..

03 March 2024 7,815 0 View

qPCR - Late amplification - Is it a quality or a quantity problem?

After trying different protocols I was able to extract RNA from old olive tree leaves with a phenol / chloroform : IAA protocol. Nanodrop measurments of the samples gave 260/280 rate ranging...

30 October 2023 7,845 4 View

Are multiple annotations on a pixel via QuPath possible?

Hello everyone! Do you possibly know if I can do multiple annotations on a pixel via QuPath? I am currently using machine learning procedure for my classifications. But what can I do if a specific...

20 May 2022 8,817 2 View

Should I change my stripping protocole or just detect b-actin as a last step (in dot blot technique)?

Hello everyone, I was doing dot blot technique for detection of Iba1 in my sample. The first time, the image background wasn't very helpful for further analysis. So, I continued with b-actin...

09 December 2021 2,445 0 View

Fatty acids-BSA conjugation can induce cell death?

Hi everyone, I am following a protocol to conjugate palmitic and oleic acid with fatty acids-free BSA. I dissolve the acids in 0.1 M NaOH to create a 100mM stock and then I add this to 5% BSA to...

07 December 2021 278 1 View

Is the Roche limit of black holes their event horizon?

As the 'Roche limit' is defined the distance within which the gravitational field of a large body is strong enough to prevent any smaller body from being held together by gravity. So, my thought...

18 September 2021 8,688 3 View

Are there available data related to the use of shared mobility for EU member states?

I wonder if there is any data platform that provides data regarding the shared mobility, e.g. use of car sharing, ride hailing, etc. for the EU member states, for free.

16 May 2021 1,082 4 View

Which analysis to use in SPSS?

I have a question about whether statistical analysis to conduct in SPSS. I have a factor that includes two levels. Also, I have multiple dependent variables with repeated measures. Which...

08 March 2021 6,093 7 View

3 Time Points with Moderation Analysis?

Hello everyone, I am currently writing my Master Thesis and I have employed a longitudinal design using three time points I am using "Coaching Alliance" (High vs Low) as an IV and Creative...

12 February 2021 8,681 7 View

Where can I find Crime Scene and Offender Data?

Hi All, I am planning my Msc dissertation and I want to find dataset(s) from crime scenes and information about the criminals linked to these crimes. Ideally, I would like personal traits as well...

23 January 2021 3,099 3 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Is there an alternative to a multinomial regression which allows the DV to be non mutually exclusive?

I am trying to analyse data from a survey examining what variables affect teachers perceived barriers to incorporating technology into their classroom. I have 5 predictor variables however my DV...

06 August 2024 1,752 3 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Do You Know Untreated TMJ Affects Your Brain And Entire Body?

04 August 2024 6,282 1 View

I need the datasets of Microgrid for system identification?

Hi I am working on data driven model of the microgrid, for that, i need the reliable datasets for the identification of MG data driven Model. Thanks

02 August 2024 5,748 4 View