Thanks for your answer. I know it works well after 30-60 min with the dye but I'm not sure I can decrease the incubation time with DHE.

Hi Carmen, I have used DHE to measure ROS by flow citometry in MEfs during 30 min of incubation after ROS exposition, and I have seen differences between treated and untreated, so, you can try in your model

In case of S.cerevisiae, DHE is working fine for 20 min incubation. So, I think you can try a test setup before going for your main experiment to validate your incubation time.

Always it's better to go with standard incubation periods (30-60min) for better results.

Good Luck..!!

Hello Upendar,

I too am using DHE in S.cerevisiae. However, can you help me whether you have used flow cytometry or any other technique to detect the fluorescence?

Hi Tarana,

I have used DHE dye to detect the generation of superoxide radical in vivo by fluorescent microscope and by Flowcytometry.

If you want to detect fluorescence by other than flowcytometry, you can go with the use of fluorescence spectrophotometer to measure the fluorescence.

Good Luck..!!