Currently planning to make hookworm protein extract with frozen adult hookworms in RNAlater solution. I am planning to first remove all of the RNAlater and wash in 1X PBS and eventually gauge peroxidase activity through an assay using ABTS and hydrogen peroxide. I did a trial run of the assay where I wanted to see if RNAlater would cleave the substrate/react in any way - in a well, I had 50/50 PBS and RNAlater, ABTS/H2O2 solution. Read OD at 405 nm; solution in the well was actually cloudy. Does anyone have suggestions as to why? Salt precipitation?
Hello! I wash the RNA later 3 times with destilled wate to my sample. Because the solution have a lot of salt. Sorry, but i don´t understad why you conserve your samples in RNA later if you extract protein?
Best regards.
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