RNA manual method - Trizol (ThermoFisher) and RNAIso Plus (Takara) what is great with Trizol over RNAIso Plus?

More Roger Francis's questions See All

Seeking Advice on Viability and Execution of Undergraduate Thesis Topic?

Hello everyone, I am currently developing a thesis proposal and would appreciate your input on its viability and how to effectively carry it out. My proposed topic is: "Does the perceived threat...

10 August 2024 8,992 0 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

What is meant by baseline of FTIR data?

I got comment on my FTIR data figure from a reviewer. The reviewer said "FTIR data in Figure should be repeated. there is no bassline." I made Y off set comparison graph of FTIR on OriginLab. Can...

03 August 2024 6,070 3 View

What is Random Audit?

HI there, I've came across several articles discuss about random audit an Non random to tax evasion or compliance. Most of the articles is relating about effect of audit (random or non random)...

31 July 2024 5,309 7 View

Is the mentioned CV graph a valid one as this graph have only one peak prominent (reduction)?

I have used Prussian blue nanoparticles as a redox couple. The PBNPs have been made using only one salt precursor. Also, during scan rate studies, a small oxidation peak can be consistently found...

31 July 2024 9,697 0 View

How we can use lattice-based cryptography for construction of S boxes?

Please suggest basic literature on lattice-based cryptography. A kind response from the cryptographic community will be highly appreciated.

24 July 2024 6,291 4 View

I obtain two plateu instead of one in LFP cathode assembled with graphite anode in 1M LiTFSI in DOL/DME, what does this mean?

I have attached the charging curve from the battery tester for review. I need help on what is happening

21 July 2024 6,898 2 View

I am working on III-V based tandem solar cells.Can anyone explain that solar cells work under forward or reverse biased conditions?

I want to know that n-doped side of solar cell is connected with positive or negative electrode.

20 July 2024 1,348 2 View

Can it is possible to find the cleaved sequence when a protein cleaved by a heamaglutanin protease (HA/P) by any bioinformatics tools?

Bioinformatics tools like peptide cutter

15 July 2024 6,453 1 View

Hello, does anyone know how I can get coordinates of two specific points on a moving body in Abaqus when applying a vdload subroutine?

Hello I am trying to simulate repulsive force of two permanent magnets in Abaqus. I have implemented Force equations into a vdload subroutine. sensitive parameters are the center to center...

15 July 2024 3,491 0 View

How to extract RNA from neurons?

Hi, I am a master's student who studies inflammation. I'm planning to extract RNA from the Vagal nerve or Dorsal Ganglion roots, but I got a question. It would be only a few 50ug per each nerve,...

23 July 2024 6,783 1 View

Size-biased recovery of total RNA isolated with Trizol vs RNeasy?

So - my lab did a bunch of RNA isolations from tissue culture cells, for purposes of RNA-seq, and submitted them to a core facility. We got back a notification that the RINs were too low, and we...

14 July 2024 641 1 View

Adding isopropanol directly to eluted RNA samples?

I have my RNA samples eluted in nuclease-free water. But now I want to store it for a longer period. Can I add isopropanol directly to my samples to store it without doing a second round of TriZol...

23 June 2024 2,576 4 View

Does Trizol LS yield more and better RNA than normal Trizol?

Hi, I am currently working on RNA extraction on blood serum samples using Trizol. However, the RNA yield is quite bad, on average, only yielded 200ng per 200ul of sample input, and the A260/280...

18 June 2024 6,854 3 View

Why do I have low 260/280 and 260/230 ratios with TRIzol-based RNA extraction?

I am extracting RNA from human adipose tissues using the TRIzol method, following the attached protocol for tissue extraction. The problem is that the 260/280 ratio of my samples is low in most...

10 June 2024 4,614 3 View

Trizol can be used for the storage of tissue sample?

Can I store the freshly collected tissue sample in trizol instead of RNA later buffer? if Yes, How long can i store? will it affect on quality/quantity of the RNA sample? Which is better among...

02 June 2024 582 3 View

How do you keep tissue cold in tissuelyser ll when you extract RNA from animal tissue?

Hi, I'm a new master's student who has been studying mouse IPF model, and I read that Trizol with tissue needs to be cold. But I can't keep the tissue cold while I homogenizing it with...

30 May 2024 8,603 3 View

Extraction of intracellular protein with TRI reagent for ELISA?

I use ELISAs for quantification of extracellular proteins in cell-conditioned media. I am interested in quantifying uncoupling protein 1 (UCP1) which is an intracellular, mitochondrial...

27 May 2024 8,025 1 View

How can I salvage a failed trizol/chloroform RNA extraction using the first interphase?

I've been working off an RNA extraction protocol using trizol and chloroform for several months that worked beautifully until I moved on to our most important samples. After completing them, I...

23 May 2024 2,475 1 View

How to increase yield and purity of RNA from muscle of EEL using TRIzol??

For my project, I need to extract RNA from muscle tissue of EEL. I am trying but the yield is low and Purity not good. The 260:230 ratio is always below 1.50 and 260:280 ratio ranges 1.6-1.86 and...

15 May 2024 3,024 2 View

Clei Endrigo Piazza

Hi! Basically the brand and price. The products are pretty the same. Tizol, Qiazol, TRI Reagent, RNAIso Plus, etc... All of them are solutions of phenol and guanidine isothiocyanate.

Best regards.

Roger Francis

Hi,

Thanks, @Clei Endrigo Piazza for the Info.

Esha Joshi

I am using Trizol (SRL) since a month but not yet getting RNA. What could the problem? Any idea?