RNA extraction from 3D spheroids?

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Nisha Rajendran

Hi Ravi,

You can store spheroids in lysis buffer at -80 for long term. Just ensure that the spheroids are lysed properly before storing them. If not lysing properly, then you can use micropestle to crush the spheroids and store them. By this method, you'll get a good yield of RNA.

Hope this helps

Thanks

Nisha

Sunil Pm

Teja, it depends on the gel for suspension you use

TriZol from invitrogen is found to good, which can ectract RNA in one hour