actually，I want to add a Fluorescent dye molecule （for example rhodamine）to a drug (such as aspirin)，But I don't known whether the Structure Modification would change the activity of aspirin，and can the altered compound enter cells?
Dear Hui,
Aspirin, acetylsalicylic acid undergoes a hydrolysis reaction in water. This reaction can be catalyzed by an acid or base. Since rhodamine contains a carboxylic acid group, in certain pH range, it may donate a proton to the carbonyl group of aspirin and thus resulting in enhancement of aspirin hydrolysis.
Regarding the permeation into cells, I do not believe that rhodamine will have any effect on the diffusion of aspirin through membranes.
Therefore, it is expected that the main problem that this approach might encounter is the hydrolysis rate accelerations of aspirin.
Hoping this will be helpful,
Rafik
Hello
i agree with Rafik Karaman , The hydrolysis rate accelerations of aspirin is the problem
see this papers may help you
Good Luck
http://pubs.acs.org/doi/abs/10.1021/ja408322b?src=recsys&journalCode=jacsat
http://www.ncbi.nlm.nih.gov/pubmed/8605980
@Rafik Karaman @Saleh Alkarim ,Thank you for your nice suggestion，it's helpful to me ,bacause I want to create a method (instead of isotopic tracer )，that can trace a compound in the body or cell.so I want to add a Fluorescent molecule to the drug ,BUT it seems difficult. So Can you give me some suggestions。
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