My question is that if we found that in the soil have some bacteria which produce PGPH then how we will find it out to know that only this bacteria have the potential ability. secondly how we will use it is a biofertilizer??
Firstly from the soil sample you have to to isolate that PGPR under controlled laboratory conditions for that you have to perform the serial dilution of your soil sample then pure culture each different colony you found over your spread plate and after that characterize each strain (from pure culture) on the basis of their PGPH production ability as described in these research articles attached below
Chapter Actinobacteria as Plant Growth-Promoting Rhizobacteria
Chapter Plant Root Enhancement by Plant Growth Promoting Rhizobacteria
@ Ahmad, first you have to isolate the individual bacteria as suggested by Dr. Vagmi, then you have to look at the individual bacteria whether they are satisfying any of the three conditions such as : tolerance toward abiotic stress through action of ACC deaminase, defense against pathogens by the presence of competitive traits such as siderophore production , increase of fertility and plant growth. Normally, PGPH comprises of following bacterial species such as Pseudomonas, Azospirillum, Azotobacter, Klebsiella, Enterobacter, Alcaligenes, Arthrobacter, Burkholderia, Bacillus, and Serratia.
thank you so much for your suggestions and articles.@Vagmi Singh@Tarafdar@ Kouadri Mohamed El Amine
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