Dear Isaac,

thanks for posting this interesting technical question. However, I think that the question would receive more qualified answers if it was a bit more specific. Most importantly, please note that pigments are generally insoluble in water and organic solvents (see e..g. the respective Wikipedia entry at https://en.wikipedia.org/wiki/Pigment). Thus you cannot analyze pigments by thin layer or column chromatography. Presumable you are talking about dyes, right? in contrast to pigments, dyes are soluble either in water or various organic solvents. In order to give you a more specific answer it would be very helpful if you could specify the individual dyes you want to analyze.

Good luck with your work!

Dear isaac, many thanks for your kind response and recommendation. Perhaps the following links might give you some initial idea:

Chromatographic separation of pigments from leaves

http://plato.acadiau.ca/courses/biol/reekie/1113/PHOTO/chromo

and

Standard Rf Values - Biology (Photosynthesis) https://www.physicsforums.com/threads/standard-rf-values-biology-photosynthesis.115321/

With best wishes, Frank Edelmann