to identify a bacterial strain by 16S, we have a similarity figure after blasting to say that the strain is of such species, so in the use of restriction enzymes digestion we can configure the result in percentage without alignment of sequence product ?? if I do not find a restriction enzyme in the gene, I can consider that the strain is from another species or a mutation
What you said is true. Still one needs to sequence and compare the amplified sequence with the known sequences to find the similarity index/ coefficient to identify the genus/ strain.
Hi there,
As a single base substitution is enough to destroy a restriction site, I don't think this criteria is enough to state on phylogeny... Or you have to show first that the full restriction site sequence is specific for one single specie.
The 16S rRNA gene sequence analysis and the ARDRA (amplified ribosomal DNA restriction analysis) as well are not sufficient to state about phylogeny. One must look for other techniques as well, such as species specific PCR assay for identification of species; RAPD would give a picture about the strain level differentiation.
What you said is true. Still one needs to sequence and compare the amplified sequence with the known sequences to find the similarity index/ coefficient to identify the genus/ strain.
- Badges
- Science topic
- Similar topics
- Biological Science
- Biochemistry
- Enzymology
- Enzymes