In disk diffusion test if my isolated staphylococcus aureus didnt show resistance to methicillin antibiotic but resistance to all other penicillin class antibiotic, dont it mean that it is not a MRSA
Is it compulsary that MRSA should show resistance to methicillin antibiotic also inspite of it showinfg resistance to other penicillin class antibiotic.
Please see this web page for clarification:
https://www.cdc.gov/mrsa/lab/index.html
According NCLSC if Staphylococcus strain is resistant to METICILIN , all penicilin and class antibiotic should be considered also resistant. Doent matter the diameter in disk diffusion.
Agreed with Antonio. I have also used MRSA strain in my experiment.
Dear ARchana
It is depend in what definition of MRSA do yu use; it should be has a mecA gene, means resistant against all beta lactam;
CLSI use OXA and now changed to Cefoxition as better predictor of mecA gene.
CLSI just predictor not absolutely true as MRSA
my experience, res to OXA or FOX is not 100% MRSA, it would be hyper producing beta lactamases.
Thus MRSA should be based on mecA gene detection
Best for you
Dear Archana, you should check resistance to FOX and the presence of mecA gene to determine MRSA.
Best regards.
Dear Archana, you can read the paper shown below for BORSA and MODSA.
Infect Control Hosp Epidemiol. 1991 Jan;12(1):14-9.
Mechanisms of methicillin resistance in Staphylococcus aureus and methods for laboratory detection.
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