I'm performing an experiment in which I inject IP a dose of o.2 mg /kg of reserpine for 14 days to produce a chronic model of depression, I want to start treatment, I read that I must give a dose each 2 days of reserpine to maintain the depressive state, does anyone knows when does the effect of reserpine normally disappears after 14 of chronic injection and in this case should I give my treatment before or after this additional reserpine dose ?