Proper RP-HPLC workup following peptide synthesis?

Hello, I've recently synthesized a short (17aa) peptide via manual SPPS. I was instructed to submit a small pipette tip's worth of material onto a capillary column to obtain a rough estimate of...

12 January 2021 9,432 3 View

SPPS: peptide failure to elongate?

SPPS of a 16 aa peptide was performed on a 0.15 mmol scale. Resin gained a minimal amount of weight: starting weight of 224 mg and a final weight of 280.I believe my peptide failed to elongate but...

21 December 2020 9,428 11 View

RNA-Seq for the analysis of ribosomal RNA (rRNA) processing sites?

Greetings! I want to study ribosomal RNA expression and potential processing sites in bacteria. To such effect, I want to perform an RNA-Seq experiment in order to analyze potential rRNA...

02 December 2020 1,310 2 View

Problems with fluorescent bioconjugation of short peptide?

I have an 18aa azido-peptide conjugated at the N-terminus (cysteine). Synthesis and folding were validated through HPLC and mass spec. Multiple attempts were made with Dylight-phosphine conjugates...

02 August 2020 2,775 3 View

Anyone with experience in virome sequencing using Oxford nanopore?

I am interested in sequencing the phages present in environmental samples (bioreators, seawater, soil...) using long reads from Oxford Nanopore sequencing. I would like to know if anyone has been...

06 April 2020 2,972 1 View

Is it possible to obtain information about the position of the atoms, taking into account the curves obtained by EDS?

In doped semiconductor samples it is very interesting to know the position of these atoms, if it is interstitial, substitutional, etc.

28 January 2020 845 3 View

How to calibrate a Pitot tube for a boundary layer wind tunnel?

Could someone please recommend me how to calibrate a differential pressure sensor for a pitot tube that will be used in a boundary layer wind tunnel, I bought a Pitot tube 160E Dwyer and a...

03 December 2019 933 3 View

R package: Pseudo r^2 in mixed effect model: which is the best approach and how interpretate?

Dear all, I am using linear mixed effect models in my experiment (using r package). I would like to show a measurement of the fitting quality of the model. I see that there are lot of methods but...

07 April 2019 1,686 11 View

What are the stages in formulating a agricultural waste management plan?

My project is about cocoa waste, I want to know both the diagnosis and the formulation of a management plan that can be applied

16 February 2018 1,691 9 View

Building a Knowledge Graph on Renewable Energy Sources?

Hello everyone: Many countries around the world are looking for updated and integrated data and information related to Renewable Energy Sources for solving practical problems in this area. The...

01 February 2018 5,152 4 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

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02 March 2021 324 2 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

APA 7th In-Text citations with two references with three authors with the same surname, but four different author?

I have an issue with APA 7th in-text citation. II have two papers where the first three authors are the same, and the fourth is different. I am using the Mendeley desktop app, and automatically it...

02 March 2021 790 3 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View