How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Is there any integrated genome browser available to see chloroplast gene expressions?

Dear colleagues, Good Day! I am looking for any public database for visualizing RNA-seq data of chloroplast genes. For ex. I want to see the expression of Polycistronic mRNA abundancies of genes...

20 December 2020 7,474 3 View

Does any one worked on vero cell line? I want to make primer but can't find sequence of Cercopithecus aethiops African monkey on any genome browser ?

vero is normal epithelial kidney cell line of african green monkey. i want to design primer but i am unable to find its name in ncbi and ensembl genome browser.

01 November 2020 7,537 2 View

How do you convert CADNANO vector drawings into .JPEG AND TIFF IMAGE FORMAT?

Hello, I am working on a DNA nanostructure but I am new to CADNANO. I need some help on how to convert CADNANO drawings into images like JPEG and TIFF. At the moment, my .svg output appears cut...

11 September 2020 4,558 2 View

Is there data on RLFS in UCSC Genome Browser?

I am currently trying to search for data on RLFS (R-loop forming sequences) for the human genome on UCSC Table Browser but I cannot find anything. Does anyone know if these data exist? Currently I...

31 December 2019 1,186 3 View

How do I design primer set for single mutation?

I have coordinates for my genes of interest and I want to design primer set to identify single mutation. I got the DNA sequences using UCSC browser and designed my primers using Primer3, however,...

15 December 2019 9,629 3 View

How do I design primer set for single mutation?

I have coordinates for my genes of interest and I want to design primer set to identify single mutation. I got the DNA sequences using UCSC browser and designed my primers using Primer3, however,...

11 December 2019 164 3 View

Best programming language for online studies?

Dear RG community, I would like to move more of my behavioural experiments online for convenience. What non-proprietary language do people recommend? A bit of background: In the past years and...

19 November 2019 1,432 10 View

How to open .bed file in UCSC browser?

I have downloaded a .bed a ChiP-seq data set from the GEO database, and whenever I try to upload it to the UCSC browser for viewing I get an error. This is the error: Error line 2 of custom...

12 November 2019 1,709 3 View

What is the best statistical tool for Android OS?

I have been working with my smartphone for a while and can complete 90% of the tasks without problem. I am really happy as I become very flexible in doing research. However I am not sure if I use...

10 November 2019 1,912 3 View

Best programming language for online studies?

Dear RG community, I would like to move more of my behavioural experiments online for convenience. What non-proprietary language do people recommend? A bit of background: In the past years and...

10 November 2019 4,346 7 View