Hi there,

I'm about to start a transfection with piggybac with an intent to generate a stable cell line. I have the transposase and transposon prepared. The selection marker is puromycin, cells are HEK293T, and transfection reagent either Lipofectamine 3000 or FuGENE6. Does anyone have a protocol I could follow for the transfection? I of course would supplement with my own particular puromycin concentration following a kill curve.

Thanks!