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1) Does accreditation or background of authors play a role in achieving a closer nutritional value to the established recommended dietary guidelines?

1) Does accreditation or background of authors play a role in achieving a closer nutritional value to the established recommended dietary guidelines for weight loss?

14 June 2024 1,322 0 View

Is it advisable to run reference genes separately from target genes in a comparative Ct analysis on ABI StepOnePlus due to logistical constraints?

Hi Everyone, I'm reaching out for some guidance regarding conducting a comparative Ct analysis with the ABI StepOnePlus system, and I hope you can help shed some light on my dilemma. Here's the...

11 June 2024 4,044 3 View

How to modelling MD of Covalent Inhibitor?

I'm designing a covalent inhibitor on an in silico study. Should I use the "pre-reaction" non-covalent ligand-protein complex or covalently bonded one on the Molecular Dynamic simulation input? I...

20 April 2024 5,662 2 View

Is this FBS debris in my cell media?

Dear all, I prepared my cell media (EMEM+10%FBS) and another cell media (EMEM+2%FBS+1% Pen/Strep). Incubate the aliquot of the media in 37°C for a few days and saw these. They are not moving and...

24 March 2024 7,496 3 View

How to dilute PVA with 31000-50000 Mw in distilled water without agglomeration?

If I stir it at high temperature, I notice a gel like layer at the top, if it wasn't heated, there are 2 layers.

05 March 2024 7,346 2 View

Via Hole Pad does not show in HFSS 3D Layout?

Hi! Why my HFSS 3D Layout doesn't show the via hole pad even when there is trace connected to it?

05 March 2024 8,296 0 View

How to assign port in HFSS 3D Layout?

Hi! How to assign port in HFSS 3D Layout for open-ended design? Can I assign the port on the via holes?

03 March 2024 1,745 0 View

I have found an article in Research gate that my name as an author, unfortunately i never publish that manuscript to anywhere. How to remove?

I have found an article in Research gate that my name as an author, unfortunately i never publish that manuscript to anywhere. How to report to remove the article? attached together is the article.

04 February 2024 9,593 1 View

How to confirm vibration mode from pySCF?

I am working to find the Transition State of a chemical reaction using PySCF. I got that conformation after Opt TS and got exactly 1 imaginary freq. how do I confirm that the negative freq is the...

01 February 2024 8,096 1 View

Mendel crossed peas having round seeds and yellow cotyledons with peas having wrinkled seeds and green cotyledons?

All the F, plants had round seeds with yellow cotyledons. Diagram this cross through the F₂ generation using both the Punnett square and forked-line methods.

12 December 2023 4,319 1 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

What are the steps of formulating a herbal extract into a sheep/goat drench?

I am working on natural remedies for de-worming in small ruminant livestocks. Part of my project involves formulation of drench-type product, which I have not much experience in. My experience is...

30 July 2024 9,806 1 View

How to maintain the drug concentration in cell culture study for long term?

I am working in the filed of long acting drugs with Nano formulation. I want to study the efficacy of my formulation in 2D cell cultures while this required media change on every two days. In such...

24 July 2024 8,696 0 View

How can I calculate the SOD enzymatic activities using the spectrophotometer method?

I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...

23 July 2024 8,492 3 View

Is there a method that I can measure alcohol dehydrogenase activity using NAD+ in enzyme extraction from plants?

17 July 2024 935 1 View

Does sucrose interfere with the BCA reagent in peptide concentration assays?

Hello everyone! I am currently working on the expression and purification of an antimicrobial peptide that experiences reduced activity after each freeze-thaw cycle. To improve its stability, I...

10 July 2024 6,830 1 View

How to dilute essential oil from plants to assess the biological activity?

I have extracted essential oil from a medicinal plant and want to make several concentrations for some biological activities (Antidiabetic assay). How can i prepare the several concentrations to...

04 July 2024 8,100 4 View

"Dear Researchers, Could anyone here share insights or tips on how to effectively draft a patent in the Nanotechnology field?

"Dear Researchers, I am currently working on a Nanotechnology project and am exploring the process of drafting a patent to protect our innovations. Could anyone here share insights or tips on how...

02 July 2024 9,627 2 View

Why should you convert a WGS to core genome? and what is a core genome?

I read a paper where they converted WGS to core genome to check for AMR through resfinder, any insights would be appreciated, new to bioinformatics.

10 June 2024 8,347 1 View

How can formulate Phosphorus 52 and Potassium 34 in liquid ?

formulate Phosphorus 52 and Potassium 34 in liquid

03 June 2024 7,589 2 View