How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Do you know of an informal caregiver of a person who has recently received a bone marrow transplant?

We are conducting a pilot study of an intervention for informal caregivers of persons who have recently received a bone marrow transplant in the US. If you know of anyone who might be interested,...

13 February 2021 9,174 1 View

Spontaneous metastasis of nsg mice reason for early metastasis?

Hello. I have injected breast cancer cells with matrigel in 4th inguinal mammary fat pad of nsg mice. i was not expecting the cells to metastasize before 2 weeks but when I imaged them on day...

31 January 2021 2,817 2 View

What is the difference between human umbilical vein endothelial cells (HUVECs) and bone marrow endothelial cells?

Hello I am new to work on endothelial cells, and I have been reading about them a lot and trying to know the difference between human umbilical vein endothelial cells (HUVECs) and bone marrow...

28 January 2021 8,765 4 View

How to gate mouse MDSC cells through flow cytometry?

On inducing the mouse bone marrow cells with X compound, there is a change in cells morphology and characteristics. In that case how to gate these cells, as these cells are showing suppressive...

27 January 2021 608 2 View

How much of cells needed to get good number of RNA ?

Hello I am growing MSCs cells in either 24 wells or 12 wells plate and then I need to lysate these cells for RNA extraction I ususally get RNA from 5 to 30s ng/ul Is there is any idea or tips...

26 January 2021 8,531 3 View

How to do rat bone Immunofluorescence, Please suggest?

Hello, I have been trying to do a rat bone Immunofluorescence histology study. But not able to find a successful protocol till now, Any suggestions? Rat bone: Decalcifies Embedding: Cryo

26 January 2021 4,165 3 View

Hello, my name is siti. My question is what is the related issue in bone marrow transplant or hematology that I can choose for my research project ?

I am doing a master in nursing but still does not have any idea about what topic I am going to choose. pls help

25 January 2021 1,097 12 View

I am culturing murine bone marrow derived mesenchymal stromal cells, what are these cells I am observing?

Dear all, I am isolating mesenchymal stromal cells (MSCs) from the bone marrow of adult C57BL/6 mice. The culture conditions are: -IMDM + L-glut, 20% of 50:50 FBS:HS, 1% P/S -Medium replaced each...

24 January 2021 7,223 3 View

How to rotate an anisotropic stiffness matrix to an orthotropic matrix of cancellous bone?

Hi everyone, recently i have a question about how to calculate the orthotropic elastic matrix of cancellous bone cube. I really need the help!!!! I applied six stress states to the cancellous...

21 January 2021 9,143 2 View

What is the best studying timeline after injection of B16-F10 melanoma metastases cell line?

I want to inject B16F10 cells into C57BL/6 mice as a melanoma metastases model to study the speed of metastases in different organs. I need advice about the designing of the best timeline for...

14 January 2021 3,704 1 View