Request for sharing plasmid?

05 May 2019 1 2K Report

Can someone share pichia pastoris pIB2 empty vector with me

I don't work with yeast but you can find it on addgene, which is a non-profit plasmid repository: https://www.addgene.org/25451/

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Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

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Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

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Why might my GFP tag be dissociating from my plasmid after transfection?

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PUC19 as a transfer plasmid for lentiviral delivery?

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28 February 2021 4,868 2 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

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25 February 2021 1,011 3 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

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25 February 2021 5,712 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

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25 February 2021 6,635 5 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

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24 February 2021 6,310 3 View