How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

Does anybody know the ways of homogeneous ripening of Mung bean crop?

Some crops (Mung bean) are matured at different times i.e., harvested at several times that's why the labour cost of the farmer is high. So, could you please, is there any ways or chemicals are...

12 February 2021 294 4 View

How much could knowing the maturity level of an organization contribute to a given process?

The doubt is raised because there are several methodologies to estimate the degree of maturity of certain processes, such as computerization, communication, human resources, among others, and...

03 February 2021 4,753 7 View

Is there any correlation between mature miRNAs and their precursors?

I have a series of RNA-seq datasets that their average spot length is about 90bp. (don't include small RNA reads like mature miRNAs). I'd like to do "Gene" and "precursor miRNAs" expression...

07 December 2020 7,744 3 View

Hello every one! How can i design the primer for mature microRNA for RT-PCR quantification?

I am going to validate some microRNA genes in two developmental stages. But i found the method for miRNA primer designing is totally change compering to mRNA and LncRNA primers designing. Please...

03 December 2020 4,555 6 View

Help in Sample Size Calculation for my Thesis Comparing the Clinical Outcome of Pulpotomy in Mature Permanent Teeth with MTA & EBRRM?

I am looking for assistance in finding statistics for sample size calculation on topic Comparing the Clinical Outcome of Complete Pulpotomy in Mature Permanent Teeth using MTA & Endosequence...

20 October 2020 8,197 6 View

MiRNA seq differential analysis?

Hello, I am writing to ask your help in regard to performing differential analysis to analyze miR RNA-seq data. I have miRNA seq data and I have analyzed in the following way- Initial QC - removed...

19 August 2020 5,105 7 View

Please i'm a new user of SAS, I need the steps to analyze a soybean data, comparing same dependent variables of 15 locations?

The design is RCBD, 16 entries, 3 reps, 4 blocks, Name. The dependent variables are date of flowering, Flower Color, Date of maturity, Daymat, Grainkha Pop_000, Dayfl, Shat2wk, Shat3wk,...

14 June 2020 571 4 View

Finding relationship between independent variables fixed per condition from a maturity model and dependent variables Likert scales from a survey?

Hello I have a question regarding a dataset I must analyze. I did two research steps: 1. Based on a managerial maturity model, I ranked 8 brands according to their level of usage of social media...

08 May 2020 7,905 6 View

Covariate in multiple regression?

I want to examine the relationship of various physical qualities (jump height, change of direction, linear sprint, etc.) on agility performance. Because I am interested in the combined effect of...

07 May 2020 3,092 10 View