I'm currently analysing some data on the effect of an agonist on calcium flux when coverslips of cells were separately transfected with different plasmids.
I'm using a 2 way ANOVA with the response to the agonist alone, the response to the agonist in the presence of an antagonist and the response again after antagonist washout being the column factors and the two plasmids being the rows factor. Whilst having the column factors as repeated measures makes sense as the measurements are from the same cells, because the cells are from the same original source before being placed to grow on the coverslips - and therefore not entirely independent -, should I also set the row factor as repeated measures?
Thanks!
Are the cells are nested within plasmid source? --- that is, Cells A, B, C, are from Plasmid I and Cells D, E, F are from Plasmid II?
The cells are transfected with the plasmids so that they then express the receptor sequence encoded by the plasmid.
Essentially a source of cultured cells was used to put cells on 3 coverslips in one dish to grow, which were then shortly transfected with one plasmid. In another dish the same source of cells was used for an additional 3 coverslips that were then transfected with the other plasmid (obviously this was repeated several times). So there are 3 coverslips transfected with one plasmid and 3 transfected with the other.
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