Hello Thomas,

it is not recommended to use DCF for measuring oxidative stress (please refer to the attached publication).

Regards, Christian

Article What does the commonly used DCF test for oxidative stress really show?

Unfortunately, I have no experience with MitoSOX at all so I can't give you advice how to best make use of it. I tried DHE on isolated mitochondria and this did not work well (highly unstable photometer readings, putative auto-oxidation problems, etc.). However, I found an interesting site where fluorescent dyes and their use for measuring ROS/oxidative stress are discussed by experts in the field:

http://oxphos.com/index.php?option=com_content&task=view&id=90&Itemid=1

I hope that this resource can be of help to you.

Regards and good luck for your projects, Christian