hi

STAT6 is a transcription factor that plays a role in the regulation of gene expression in response to certain cytokines and growth factors. Phosphorylation of STAT6 at Tyr641 is important for its activation and function.

Downregulation of pSTAT6 (phosphorylated STAT6) can affect mRNA expression in a number of ways. For example, pSTAT6 can bind to specific DNA sequences called STAT6 response elements and regulate the transcription of target genes. If pSTAT6 levels are reduced, this could result in a decrease in transcription of target genes.

It is also possible that pSTAT6 could have indirect effects on mRNA expression through other signaling pathways. For example, pSTAT6 could activate or inhibit other transcription factors or signaling proteins that regulate gene expression.

It is not uncommon for the effects of signaling pathways on gene expression to be complex and multifaceted. The specific mechanisms by which pSTAT6 downregulation affects mRNA expression in your particular experiment will depend on the specific cell type, signaling pathways, and target genes involved.

The extent to which pSTAT6 downregulation affects mRNA expression may depend on the specific target gene. Some genes may be more sensitive to changes in pSTAT6 levels than others, and the degree of pSTAT6 downregulation may also play a role.

The duration of pSTAT6 downregulation could also affect mRNA expression. For example, a 20% reduction in pSTAT6 levels at 30 minutes may not have the same effects as a sustained reduction over a longer period of time.

The presence of other signaling pathways or transcription factors could also influence the effects of pSTAT6 downregulation on mRNA expression. For example, if other signaling pathways or transcription factors are able to compensate for the loss of pSTAT6, this could mitigate the effects on mRNA expression.

It is also important to consider the specific cell type and tissue context in which the experiment was conducted. Different cell types and tissues may have different requirements for pSTAT6 signaling and may be more or less sensitive to changes in pSTAT6 levels.

Thanks for your informative response.

And to make to clear I used airway epithelial cells in vitro and the stimulation was done by using IL-13. What I also found in literature is that the peak of STAT6 phosphorylation is at 30 minutes of stimulation.