Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View

How to separate epithelial cells?

Hello everybody, Hope you all are doing fine during this difficult time. I am trying to culture mouse oviductal epithelial cells but, the problem is when I culture them there is a mixture of...

31 October 2020 2,640 4 View

Are ExpiSf9 cells exclusive for suspension culture?

Hi All, It is recommended by Thermo Scientific to used ExpiSf9 cells as suspension culture. I am wondering if anyone has experience with other than suspension ExpiSF9 cell culture to produce...

12 February 2020 4,463 3 View

Is deproteinization must do step while measuring lactate in blood plasma?

Greetings, I am trying to measure L-lactate in mice blood plasma. For that, Is it absolutely necessary to remove protein form plasma sample before measuring lactate?. How much does this affect the...

09 February 2020 7,752 3 View

Why there is no more binding of protein after recharging the his trap column?

I want to purify the protein from E.coli. My proetin of interest is expressed in pellet . so i used lysis buffer( 50mM tris, 6M GnHCL, 20 mM imidazole and 500 mM nacl),. After stripping , clean up...

09 February 2020 9,394 7 View

Is there any protocol available to construct baculovirus based plasmids?

Hi, I have been using bac-to-bac system for protein production using Sf9 and/or Tni cells. I am just curious to know if it is possible to construct baculovirus based plasmids? Any suggestion is...

26 December 2019 4,636 3 View

Which is the best somatic cell for mice embryo co-culture development in vitro?

Greetings, I am trying to do an autologous coculture for mice embryo development in vitro. But, I am unsure which somatic cell is best for the development (like Cumulus cell, MEF, OECs, etc.). Can...

22 December 2019 1,697 1 View

Is it possible that weight of fly ash concrete cubes is less than pure OPC concrete cubes after 90 days of curing in tap water but strength is more?

I m comparing compressive strength of fly ash based concrete and OPC

09 October 2019 5,876 3 View

Can anyone send me "Testing code of concrete for port and waterwog engineering(JTJ 270-98)?

i want to know how water soluble extraction of concrete blocks powder will be performed?

05 August 2019 8,001 3 View

Is there any relation between compressive strength and flexural strength for RHA concrete?

sir, Is there any relation between flexural strength and compressive strength in RHA concrete?

14 July 2019 3,486 4 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

No title

03 March 2021 5,360 2 View

How can we raise the figure when we writing a paper with Latex ?

Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...

26 February 2021 3,738 3 View

What is the best way to calculate the angles of twist for concrete columns subjected to torsional moment using finite element software?

In Finite element software like ANSYS , there is difficulty in obtaining direct results of twisting of concrete members because SOLID65 which is the unique element designed to represent concrete...

26 February 2021 3,806 2 View

Compute for the total volume of concrete needed for the concrete pouring of the foundation including its column up to NGL only as shown in the plan?

Looking for the quantity of cement, sand, and gravel of footings and columns. Thank you!

25 February 2021 3,092 2 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Can I use TCA instead of TFA for protein analyis in RP-HPLC?

I am trying to analyze lipase using the reverse phase-HPLC C18 column. In the literature, 0.1 % TFA was and 60% ACN was used. Can I use TCA in place of TFA? If any other methods are available to...

25 February 2021 2,388 3 View

Can I use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography?

Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...

22 February 2021 730 2 View

My silica gel column dried can I somehoe save the trapped matter?

I tried to separate my substance from a protein in a sillica gel column. By mistake, it dried and cracked. Can I save the trapped substance? Can I repack it ,or at least elute all of the trapped...

22 February 2021 1,789 3 View

Physical-chemical formation of stalactites/stalagmites. what promotes the formation/growth of these structures?

I know, or think I do, the basic idea behind these natural structures, as from what I know they form due to water dripping from the top leaving a salt deposit behind. But started to wonder about...

22 February 2021 1,400 4 View