Seema Bharatiya

3 Questions 8 Answers 0 Followers

Questions related from Seema Bharatiya

Regarding generation of His trap column?

I regenerate my column with using the process- 1- 3 CV wash with Mill q water 2-wash the column with 3CV of 0.5M EDTA of pH-8 3-again wash the column with 5CV of Mill q water 4- regenerate the...

09 September 2020 6,719 1 View

Why there is no more binding of protein after recharging the his trap column?

I want to purify the protein from E.coli. My proetin of interest is expressed in pellet . so i used lysis buffer( 50mM tris, 6M GnHCL, 20 mM imidazole and 500 mM nacl),. After stripping , clean up...

10 February 2020 9,485 7 View

No colonies formation after transformation - what are the possible region ?

I am trying to clone p50 and p65 in pet 32a , doing pcr with for p65- using the Bam h1 and hindIII and for p50 -using the sites EcorI and HindIII , get the amplification , digest the DNA by using...

01 January 1970 5,189 0 View