Coplymer of PHA contains 3hb and other units. crotonic acid assy can quantify only 3HB, CAN ANYONE PLEASE help in understanding if we can use this method to quantify copolymer.
The crotonic acid assay is a method commonly used to quantify the content of 3-hydroxybutyrate (3HB) in polyhydroxyalkanoates (PHAs). However, as you have correctly mentioned, copolymers of PHA contain other units in addition to 3HB, and the crotonic acid assay will only give you an estimate of the 3HB content.
To quantify copolymers of PHA accurately, you need to use a more comprehensive method such as nuclear magnetic resonance (NMR) spectroscopy or high-performance liquid chromatography (HPLC). NMR can provide a detailed analysis of the composition of PHA copolymers, including the identity and relative proportion of the different monomers. HPLC can also be used to separate and quantify the different monomers in the copolymer.
Therefore, it is not recommended to use the crotonic acid assay to estimate or quantify copolymers of PHA, as it will not provide an accurate measurement of the total polymer content or the composition of the copolymer.
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