Hi:

I am running fluorescence assay for the first time, the excitation and emission wavelength of my compound consecutively are 300-410 nm, while the plates in my lab (white clear bottom plates) enable reading down 340 nm only. My question is could I use those plates for my experiment while the emission wavelength is out of the plate's reading range? I mean is it supposed to use a plate with wavelength reading's range including excitation and emission or emission only?

I am using CLARIOstar® device to run this experiment. I tried my best to look online to find plates which allow reading up to 410 or more but I could not find any. According to the original assay article, they used acryl disposable cuvettes, which is not practical for my assay as I have many dilutions for many samples and controls and I do not have a device can be used with cuvettes for fluorescence assay.

Thank you

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